H2O (10%)2 ml(NH4)2SO4 (10%)1 mlCu(NO3)2 (15%)1 mlFeSO4o7H2O (0.05%)1 ml Figure 2 - Reagents usedReagentStock concentrationFinal concentrationCalculated stock volumeStreptomycin50 mg/ml200:g/ml2.4 mlDL- Threonine1% in H2O10 ml/L6 mlL-Leucine2% in H2O10 ml/L3 mlL-Proline2% in H2O10 ml/L3 mlL-Histadine1% in H2O5 ml/L3 mlL-Arginine6% in H2O33 ml/L.33 mlVitamin B10.1% in H2O0.2 ml/L1.2 mlGlucose40%10 ml/l1.5 ml Figure 3 - Colony countsThreonine deficient platesTime10-110-210-310-4 0 minutesTNTCTNTC961710 minutes41TNTCTNTC12720 minutes385TNTC14830 minutes884TNTCTNTC40 minutes216TNTCTNTCTNTC50 minutes345TNTC4260 minutesNG6TNTC53Proline deficient platesTime10-110-210-310-4 0 minutesNGNGNG310 minutes37NGTNTC4420 minutes49TNTCTNTC5830 minutes43TNTCTNTC4040 minutes115TNTC9450 minutes53TNTC505160 minutes14NGTNTCNGHistadine deficient platesTime10-110-210-310-4 0 minutes102NGNG10 minutes464326320 minutesTNTC136NG430 minutesTNTC1848340 minutesTNTC34020NG50 minutes45TNTC27360 minutes4125042NGTNTC = Too numerous to count NG = No growth Figure 4 - Time of entry table (percentages of recombinants)TimeThr -Pro -His -0 minutes.440010 minutes5.82.0220 minutes6.72.6.0630 minutes.0041.8.0840 minutes.00984.3.0950 minutes1.92.3.00260 minutes2.40.11 Figure 5 - Time of entry graph (percentages of recombinants) Figure 6 - Map of E. coli K12...
