ltsHexane- 0Hexane 90:10 (All 4 samples)- 0Hexane 80:20 (Both Samples)- 0Triolene- 85/149= .570Oleic Acid- 55/149= .369Cholesteryl Linolet- 150/150= 1Crude Lipid- 15 spots IV. Discussion In this experiment, extracted lipids are compared to three known lipids listed above. The results obtained for our group were classified as incorrect due to unknown experimental error. Therefore, results from another group were attained for discussion purposes. In looking at the results it cannot be clearly identified what the unknown lipid is. The Rf values from the three standard are triolene: .78, oleic acid: .309, and cholesteryl linolet: .99. The crude lipid produced 10 different spots. Spot 9: .669 is fairly close to the standard triolene. On the crude results, spot 10: .99 is close to the standard cholesteryl linolet. The rest of the hexane 90:10 and 80:20 samples did not match up to the standards. The hexane was supposed to produce a 0 Rf values since it is nonpolar and would not be expected to travel on the TLC plate. Theoretically, the hexane extract, a very nonpolar compound, should have traveled further than the 90:10 and 80:20. In looking at the results this is exactly what happened since the final value of the lipid was .99. The Rf value of the hexane extract should be near one as those of cholesteryl linolet or oleic acid because the silica gel is polar. The 80:20 extract is the most polar extract as far as this experiment is concerned and it should have the lowest Rf value since it will bind to the silica gel plate and will not travel with the solvent front as readily as nonpolar molecules. The 80:20 should have Rf values near the oleic acid, since both are polar. As for the 90:10, the Rf value does not suggest that it is any of the standard lipids, however, during the visual inspection, its location appeared to be in the same position as triolein. The Rf value of triolein and that of the 90:10 extract are the nearest ...
