placed in a water bath and incubated at 37 degrees Celsius for 30 min. The incubated samples were placed in the model 340 spectrophotmeter and read at 562 nm. The readings were taken, and the protein amounts were calculated using a standard curve( y = .0065x + .0146). We calculated the volumes needed of each sample to get equal amounts of protein in each well on the gel. SampleAbsorbance, 562 nmug Proteinul to load on gelBlank0C.27339.7510.94LSS.24435.2912.24HSS.19928.3715HSP.27339.7511SDS PAGE We were given a pre-cast SDS page gel(8-16% tris-glycerine gel, 1.00 mm X 12 well). The gel was labeled with the two sets of cell fractions and a MW marker( Bio Rad SDS PAGE standard.) A purified RUBISCO sample was loaded as a control. The samples were loaded into the apparatus( Novex Experimental Technology EI 9001 X cell II minicell). The gel was run at 170 volts( different form manual) for one hour. The gels were run and picture was taken....
