el and place it in the electrophoresis box with the wells towards the cathode end. Pour the prepared 1X TEA buffer carefully over the gel until the liquid level completely covers the gel and is about 1 or 2 mm above the surface of the gel. Add 2 l of loading dye to each of the enzyme tubes using the micropipet and spin them in the centrifuge. Extract 10 l of the first sample and load it into the first well. Repeat this with the other samples, changing tips between each.Attach the power supply to the electrophoresis box. Set it to 100 volts and 40 milliamps and activate it. After about 45 minutes or until the dye is approximately of the down, turn off the power supply and disconnect the box. Using gloves, remove the gels from the box and place them on the transilluminator. The instructor will carry out the photography of the electrophoresis gel.Clean the lab area.IV. Observations and ResultsHindIII EcoRI BamHI Control Distance Act. BP Distance Cal. BP Distance Cal. BP Distance Cal. BP3.4 cm25,000*3.5 cm23,0003.8 cm19,0003.7 cm20,0004.89,4165.37,8004.215,000------------------------5.9 6,5576.45,2005.76,800------------------------6.74,3617.14,0005.96,500------------------------11.32,3228.73,3006.74,300------------------------12.12,027-----------------------------------------------------------------------* = rounded base pairAll calculated base pairs (Cal. BP) are rounded figures.V. ConclusionsElectrophoresis literally means "to carry with electricity." It is the use of restriction enzymes and electrical current to measure segments of DNA from a sample.Restriction enzymes are enzymes found in bacteria. These are enzymes that are able to cut through the phosphate-sugar backbone of DNA at restriction sites. Restriction sites are certain base sequences recognized by these enzymes. In bacteria, restrict...
