is taken from the suspect and DNA is spooled from it. The same restriction enzyme is added to it, and both samples are run through electrophoresis. Since every single person has different genetic material, a match in segments between restriction sites would be an impossibility to be classified pure coincidence. This would clearly identify the suspect as the perpetrator. A difference in segment lengths would clear the suspect, as the DNA would be clearly different. In our electrophoresis experiment, it is shown how 3 different restriction enzymes act completely differently on the same sample of DNA. This is because each enzyme has a different restriction site it acts upon. The control in this experiment simply shows that DNA without any cuts would run, but would run as a large clump and would run very slowly, as it cannot maneuver easily through the gel matrix.VI. Questions2. Restriction enzymes are enzymes that use DNA as a substrate. When the proper base sequence, called a restriction or recognition site, is found the enzyme acts by cutting between the backbone two specified bases.3. Restriction enzymes are found naturally in bacteria. They act as a protection against viral infections, as they break down incoming viral DNA.4. The electricity in electrophoresis acts on DNA as a magnet does to another magnet. DNA has a slightly negative charge. The samples containing DNA are loaded at the cathode or negative end. When the power is activated, the DNA is attracted towards the positive end of the electrophoresis box. The agarose gel provides a means of slowing the DNA down. The DNA fragments must work through the gel matrix in order to reach the end.5. 6. The loading acts as a point of reference. It allows the person performing the experiment to see how far down the DNA sample has moved. The DNA is photographed using ethidium bromide, a UV-sensitive substance and an ultraviolet transilluminator to highlight the DNA strands...
