y to hemolyze red blood cells to obtain the iron found in them. There are three types of hemolysis: gamma lysis, alpha lysis, and beta lysis. Gamma lysis is the absence of erythrocyte lysis showing no color change in the blood agar. Alpha lysis is a partial break down of hemoglobin and results in a color change from red to green. The third kind of hemolysis is beta lysis, which is the total degradation of hemoglobin and shows a color change from red to clear.The second test is on mannitol salt agar. This test is selective for those bacteria able to survive an environment containing high salt concentrations. The test also shows whether or not a bacterium has the ability to ferment mannitol. The medium contains a pH indicator, if mannitol is fermented then pyruvic acid is produced which drops the pH. If this happens a yellow halo will appear around the bacteria growth.The coagulase test checks for a bacteria’s ability to clot serum. If the serum clots then the bacteria possesses coagulase activity. The tests were performed and placed in incubators. The results were checked 48 hours later.Week 2, Day 2All tests were viewed and the results were recorded. Analysis of the results was made and the bacteria were identified. ResultsWeek 1, Day 1After the initial gram test was complete the bacteria found in unknown #10 were determined to be as such: The first bacteria viewed will be referred to as Unknown 10a and the second will be Unknown 10bUnknownGram stainMorphology10aNegativeSingle rods10bPositiveClusters of cocciWeek 1 Day 2The optimal growing temperature for both unknown bacteria was found to be 37 degrees. The next series of gram stains showed that the isolation was successful. Both Unknown 10a and Unknown 10b were isolated. The isolated colonies were transferred to slants and stored over the weekend.Week 2, Day 1The slants of Unknown 10a and Unknown 10b were again tested for total isolation using the gram staining ...
