g itself to the active site (competitive inhibitors) blocking the substrate; (2) by attaching itself to the enzyme in a place other than the active site, and changing the shape of the active site, thus disabling the active site. In this experiment, some of theses factors will be tested, concentration, heat, and pH levels. Much insight can be gained into their affects upon the rate of reaction. This experiment should show what the optimum concentration of the enzyme and pH level, and temp that is needed for the enzyme to perform at it’s peak rate.Methodology:This experiment can be divided into three related sections, the testing of concentration, pH, and temperature. The concentration was first to be tested. Four test tubes were labeled one to four respectively, and 3ml of 3% concentration H202 was added to each. Test tube one received one drop of the enzyme, then was shaken for twenty seconds. The test tube was then plugged and the rate of reaction was tested by measuring the gas pressure inside the test tube for three minutes. Test tube two received two drops of the enzyme, once again shaken, then gas pressure was measured. This process was repeated for test tubes three and four, bot of which receiving three / four drops of the enzyme solution respectively. The next section of the experiment tests the effect of temperature upon the rate of reaction. Once again, four test tubes were labeled (one to four); each receiving 3ml of 3% concentration H202. A tub of water was cooled to a temperature between 0-5 Celsius. The first test tube containing 3ml of H202 was then dropped into the water to be cooled to this temperature also. Upon reacting the 0-5 Celsius temperature inside the test tube, two drops of the enzyme solution was added to the test tube, and shaken for twenty seconds. Then the gas pressure was measured inside the test tube as before. This process was repeated for each of the remaining test tubes, except tes...
