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Enzyme Action Testing Catalase Activity

t tube two was measured at a temperature of 20-25 Celsius, test tube three was measured at 30-35 Celsius, and test tube four was measured at 50-55 Celsius.The final section of this experiment tested the effect of pH upon the organism. Three test tubes were labeled respectively, and 3ml of 3% concentration H202 was added to each. Then, an additional substance was added to each of the test tubes, 3ml of pH 4 (for test tube one), 3ml of pH 7 (for test tube two), and 3ml of pH 10 (for test tube three). Then two drops of the enzyme solution was added to the test tubes, shaken for twenty seconds as before, and once again the gas pressure was measured for three minutes. This was repeated for test tubes two and three.Results:Data that was collected for this experiment on the rate of chemical reactions is summarized in the following tables, and the graphs attached.Table 1 Enzyme Concentration 1 drop 2 drops 3 drops 4 drops Rate of Reaction (atm/min) 0.9 1.2 3.6 4.8 Table 1 summarizes the rate of reaction of the enzyme upon the substance (H202) by comparing different concentration levels of the enzyme, ranging from one to four drops. One can reach the conclusion that the higher the concentration, the higher the rate of reaction.Table 2 Temperature Range (Degrees Celsius)Actual Temp. In Test Tube (Degrees Celsius)Rate of Reaction (atm/min)0-53.5-0.120-2523.60.630-3531.05.150-5552.1-0.5Table 2 summarizes the rate of reaction of the enzyme upon the substance (H202) by comparing different temperature conditions of the enzyme, ranging from 0 to 55 Celsius. One can reach the conclusion that the optimum temperature of this enzyme is 30-35 Celsius. Each of the other temperature ranges produce results of either negative amounts or insufficient reaction rates. Table 3pH Buffer LevelReaction Rate (atm/min)pH 40.7pH 72.5pH 103.7Table 3 summarizes the rate of reaction of the enzyme upon the substance (H202) by comparing different pH levels the enzyme...

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